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The medium was aspirated the next day (24 h after seeding) and was replaced with the experimental culture media, and cells were allowed to equilibrate for 1 h in DMEM (Gibco), and supplemented with 10% dialysed fetal bovine serum (FBS), 1% penicillin/streptomycin, 5.6 mM glucose, and 2 mM l-glutamine before change of media and addition of APAP

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Cost and convenience differences are substantial between administration methods