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Needle gauge matters too: a finer needle (commonly 29-31 gauge for insulin syringes) reduces the mechanical stress placed on the solution as it passes through the needle bore compared to a larger-bore needle, and produces a smaller, cleaner puncture through the vial's rubber stopper, reducing the risk of coring small rubber fragments breaking off into the solution

However, keep in mind that we lack long-term data and large peer-reviewed studies on berberine's effects on organ function and overall health over extended periods of time