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the antibody affinity for viral epitopes regulates the fraction of epitopes on the viral particle occupied by antibodies at any given concentration that is referred to as occupancy that predominantly determines the neutralization potential, as well as the accessibility of epitope, are both obligatory to exceed the threshold requirements as the antibodies possessing poor accessibility to specific epitopes require a higher concentration to exceed the occupancy threshold for neutralization 36,76
Mistake 1: Using your old concentration chart You switched from 10mg/ml to 20mg/ml but kept following the same chart from your previous vial
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Reasonable, untested
