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Bi-weekly monitoring of absolute food intake and body weight showed a continuous increase in body weight across all DIO mice groups, including those treated with saline or fed a normal diet, indicating a sustained weight gain trajectory throughout the experimental period (Supplementary Fig
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Glucagon-like peptide-1 (GLP-1), a gut-derived peptide hormone, plays an important role in transmitting appetite signals from peripheral to central feeding-related nuclei via sympathetic and vagal pathways [12, 13]
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