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Yamanouchi D
J Inflamm (Lond) (2016) 13:36.10.1186/s12950-016-0145-0 35 DurusoyMKaragzEOztrkK
The MSIA tips are then rinsed with a series of buffer and water rinses to remove salts and detergents ( Figure 1 , step 3), after which captured IGF-1/LR3 are eluted from the MSIA tips by iteratively pipetting an elution solvent of 33% acetonitrile in 0.4% trifluoroacetic acid, TFA (step 4)
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