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For Salmonella infections, immortalized BMDMs were seeded in 12-well plates (4 10 5 cells per well) in RPMI 1640 supplemented with 2 mM L-glutamine and 10% FBS at 37 C and 5% CO 2 for 24 h
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When MIC B12 Injections Can Help Confirmed B12 deficiency: Injectable B12 is a standard medical therapy and can be important when absorption is impaired (NIH ODS overview: Vitamin B12 fact sheet )
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