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To quench the metabolism, 500 l of precooled -48 C 100% methanol (Sigma, Germany) was added to the cells, which were then incubated for 30 minutes at -80 C

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Our study also delivers, to the best of our knowledge, the first near-atomic-resolution structure of a PV1 sVLP in complex with a potently neutralizing, D-antigen-specific antibody