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Endpoints range from EEG-derived sleep-stage proportions in restrained and unrestrained animals to plasma hormone panels, behavioral pain assays and opioid-interaction protocols

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The absence of IGF-1 stimulation is particularly relevant for long-term safety

We then used a Cryostat Microtome CM3050S (Leica, Germany) to section the muscle sample, the tissue sections were stored at 80 C for later Hematoxylin and Eosin stain (H&E stain), Gmri trichrome stain, and nicotinamide adenine dinucleotide dehydrogenasetetrazolium reductase (NADH-TR) stain as described previously 28