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The post-opening discard period exists because: Sterility can no longer be fully guaranteed after first puncture Preservatives slow microbial growth but do not eliminate contamination risk Risk of contamination increases with repeated vial access For this reason, healthcare and laboratory settings typically follow a fixed discard timeframe (commonly 28 days), unless otherwise specified by the manufacturer

PMC / Aging Cell (2025) PMC12727671 Confirmed age-related NAD+ decline across multiple species (rodents, nematodes, and select human tissues including skin, liver, and brain)

Scientists have noted that the simplicity of the molecule gives consistent results across different experimental conditions and is stable under standard lab storage conditions

Because the body does not produce B12 naturally, maintaining healthy levels through diet or supplementation is essential