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Depending on the plate layout, either LPS (1 g/ml) or ConA, (2.5 g/ml, Sigma-Aldrich, Steinheim, Germany) was added in four replicates per cow in addition to four unstimulated wells (with Seahorse medium for volume compensation)
Raun K, et al
The manufacturer's guidance, aligned with MHRA-approved prescribing information , provides clear instructions for these situations to help you maintain safe and effective treatment
The decision to increase should always involve your healthcare provider
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