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For signal detection, the ABI Prism 7700 sequence detector (Life Technologies, CA, USA) was programmed to execute an initial step of 2 min at 50C and 10 min at 95C, followed by 40 thermal cycles of 15 sec at 95C and 1 min at 60C
See the reconstitution guide for preparation steps
10.1371/journal.pone.0215277 PLoS One 37 CanoE.MahadevanL
A SNP is essentially a mis-programming in the building of a protein (usually enzymes) so that one amino acid is placed where another one should be
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