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it is essential
This process requires the involvement of protein disulfide isomerase (PDI), ER redox protein 1 (ERO1), and GRP78 chaperone protein
Fortunately for those of us with hemochromatosis, this research established that one of the many medicinal properties of turmeric is to reduce excess iron from both the bloodstream as well as the cells of the body
So if you know your protein of interest is happy in another buffer, you can likely use that for the binding buffer
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