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Brain and body tissue samples were transferred to Seqmatic, a local single cell scRNA company based in the Bay Area, for nuclei extraction, fixation, barcoding and library preparation using a Parse Megakit

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Additional randomized, controlled trials that follow people over time are needed to confirm these findings, but these results are promising, since people with diabetes are at increased risk for developing epilepsy later in life, said study author Edy Kornelius, MD, PhD, of Chung Shan Medical University in Taichung, Taiwan

The membrane was washed with TBST, then incubated at room temperature for 2 h with horseradish peroxidase-labeled goat anti-rabbit secondary antibody (A0208, Beyotime, Shanghai, China) and goat anti-mouse secondary antibody (SA00001-1, Proteintech, Wuhan, China)