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Thus, a GST 4QN fusion tag is recommended to prevent aberrant pulldown experiments
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GLP-1 plays several critical roles in glucose and appetite regulation: it stimulates insulin release from the pancreas, inhibits glucagon secretion to prevent excess glucose production, slows gastric emptying to moderate post-meal blood sugar spikes, and promotes satiety by acting on hunger centers in the brain (6)(23)
The membrane was then solubilized with 0.5% (w/v) lauryl maltose neopentylglycol (LMNG, Anatrace) and 0.1% (w/v) cholesterol hemisuccinate (CHS, Anatrace) with additional 2 M tirzepatide for 3 h at 4 C
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