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We found the structure to be very similar to the published binary complex of Pt GSTF8 with GSH (PDB 5F07) 39 at an overall root-mean-square deviation (RMSD) (C) of 0.16 (Fig

The cells were cultured in Eagles minimum essential medium (ThermoFisher Scientific) or DMEM F-12 (ThermoFisher Scientific) respectively, supplemented with 10% Fetal Bovine Serum (Gibco) and 1% penicillinstreptomycin (ThermoFisher Scientific), in a humidified incubator maintaining 5% CO 2 at 37 C