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Following endogenous growth hormone release, downstream production of insulin-like growth factor-1 (IGF-1) contributes to many of the physiological processes associated with growth hormone biology
The reaction between RT and GSH is based on a Michael addition reaction that is inherently reversible, has an appropriate dissociation equilibrium constant K d in the mM range and rapid reaction kinetics, and provides ratiometric readouts, thus allowing GSH quantification independent of the probe concentration
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