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Angle the needle against the inner wall of the vial and push the plunger slowly

This is problematic because: Imprecise measurements: 1 ml on a 10 ml syringe is difficult to measure accurately

We noticed that SsCm1 does not contain the recognizable cTP, but it can be partially localized in chloroplasts, and artificially expressing SsCm1 in chloroplasts can more effectively inhibit elicitor-induced ROS accumulation and cell death, indicating that SsCm1 mainly functions in chloroplasts

The current views ascribe fundamental function to chloroplasts in orchestrating defense responses: upon plasma membrane sensing biological threats, signals are quickly relayed to chloroplasts through the plasma membrane/chloroplast dual-located proteins or calcium signal 5,7