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Ligand-binding Dectin-1 activates phospholipase C via phosphorylation and then activated phospholipase C generates inositol trisphosphate and diacylglycerol for triggering an intracellular Ca 2+ flux in dendritic cells (173)
For the mitochondrial OCR assays, mitochondria were plated on Seahorse Biosciences 96-well culture plates (5 g of protein per well) and centrifuged for 20 min at 2,000 g at 4 C to adhere mitochondria to the base of wells
The redox framework is a powerful clinical tool, but it is a window into systemic biology, not a closed therapeutic loop
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