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Practical considerations include keeping the peptides in separate vials

doi:10.1016/j.jacc.2017.09.1085

Co-culture models that combine distinct hiPSC-derived cell types to replicate key aspects of the BBB exhibit physiologically appropriate characteristics including tube formation, organized tight junctions with acceptable transendothelial electrical resistance (TEER), expression of active transporters, and uptake of low-density lipoprotein (Lippmann et al., 2012