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CDC summarizes USP guidance: date an opened multi-dose vial at first puncture and discard it within 28 days unless the manufacturer states another opened-vial date. The dose count and 28-day window together require three vials for the 12-week protocol

Individual differences: The duration of B12 injection benefits can also vary based on individual factors, such as age, genetics, and overall health status

Visceral fat likely decreases at a slightly faster rate than subcutaneous fat, consistent with the higher beta-3 receptor density in visceral adipose tissue

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