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After incubation for 30 min at 37 C, samples were shock-frozen in liquid nitrogen
Supernatants were discarded, and water lysates were combined with the tube contents
This method allows for better absorption compared to taking oral supplements, especially beneficial for those with absorption issues.
Nestin, an intermediate filament protein, is associated with neural progenitor cells and is typically expressed during early stages of central and peripheral nervous system development
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