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RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR
Perhaps the best single paper on this topic comes from Oprea and colleagues and was published in the British Journal of Anaesthesia in July 2025
To some extent, the phenotype correlates with genotype for VLCAD deficiency [123]
Leggio L et al
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