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PubMed Jastreboff, A.M., Aronne, L.J., Ahmad, N.N., Wharton, S., Connery, L., Alves, B., Kiyosue, A., Zhang, S., Liu, B., Bunck, M.C., Stefanski, A., & SURMOUNT-1 Investigators
His-C16orf87 and GST-HDAC1 protein purification Plasmids encoding different 8 His-C16orf87, GST, and GST-HDAC1 proteins were transformed into BL21(DE3) competent cells, bacterial cultures were grown to OD 600 = 0.5, and protein expression was induced with 1 mM IPTG at 37 C for 3 h
Immediate effects may include increased hydration, better mood, and mental clarity
