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A gradient program was run from 90% solvent A (water) to 90% solvent B (acetonitrile) in 10 min and the final condition was held for 10 min

We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )

In monomeric form of DPP4 has no enzyme activity and is only fully active as a homodimer