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The proteome concentration of the tissue lysates was determined using the DC protein assay (Bio-Rad), normalized to 2 mg/mL, and then 100 L of each sample was transferred to a LoBind Eppendorf tube containing 48 mg of urea
It is attributed to the presence of certain nutrients such as omega-3 fats, vitamins, polyphenols, and cocaine, as well as foodstuffs such as fish, nuts, seeds, vegetables, fruits, coffee/tea, and fermented goods, which are currently being studied
2006 Dec;91(12):5013-8
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