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First, in the process of disrupting the IGF-1/IGFBP complex by acid/ethanol precipitation, there is the potential IGF-1 loss, which would directly affect the measurements accuracy
Thermoprecipitation of Glutathione S-Transferase by [PMID: 21567582] Laboratory literature summary: Thermoprecipitation of Glutathione S-Transferase by Glutathione-Poly(N-isopropylacrylamide) Prepared by RAFT Polymerization
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