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IGF is a central player in changing the fate of cells during survival and proliferation through the modulation of leading signaling pathways, including Jak/STAT, MAP kinase/ERK, and PI3K/Akt, and subsequent impacts on steroidogenesis and cell division
Horvth JE, Groot K, Schally AV
10.1016/j.biopsych.2012.01.014 24
Extracts were cleared by centrifugation at 15,000 g for 15 min at 4 C, and supernatants were incubated with 2 g of purified GST-tagged GFP-Nanobody for 30 min on ice
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