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As this region of ORC1 cleaved by X(PC4) variants occurs in a section of the protein predicted to be structured and therefore may be less accessible to a protease, we prioritized eliminating cleavage of IL-1 by performing evolution using dual-selection PANCE with a 25-amino-acid segment from IL-1 as the negative selection substrate, IL-1 (residues 529
Stefano Sinicropi , Founder of HyperCharge Health and HyperCharge Wellness Clinics Disclaimer: This blog is for informational purposes only and is not intended as medical advice
doi:10.2147/IJGM.S301900 [PubMed] 126
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