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As can be seen from Figures 4 , the samples of NC, FI and GSH groups are all in the 95% confidence interval, and can be spatially separated uniformly without overlap, indicating that there are significant differences in metabolites between the two groups, and that they are able to be analyzed in the next step

Gut microbiota analysis revealed distinct microbial structures across groups, with the UC + low-BBR 14 days group showing significantly higher relative abundances of Bacteroides , Alistipes , and unclassified_Clostridia_vadinBB60_group compared to the UC group ( p 1, and the Wald ratio method was applied when SNPs = 1