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Senescent tumor cells secrete a TGF--enriched senescence-associated secretory phenotype (SASP), which activates AKT-mTOR signaling, reshapes the TME, and promotes malignant progression under therapeutic pressure

Additionally, factors such as stress, poor nutrition, and environmental toxins further deplete our levels of glutamine and glutathione

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Then, glutamate (10 mM) and succinate (10 mM) were added to induce CI and CII substrate-linked respiration