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However, due to purification of the K194A-mutated CGL, all the buffers used were supplemented with 1 mM PLP and 10 mM l-methionine to prevent the protein precipitation, which was probably caused by the dissociation of the tetramer
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This characteristic curve shown in Figure S1B indicates that TPE-DCV is a typical AIE active fluorogen with an f w threshold of 68%, above which the dye molecules aggregate and the emission is turned on
