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Normal human primary hepatocytes (ThermoFisher Scientific) were thawed in the Cryopreserved Hepatocyte Recovery Medium, centrifuged, then resuspended and plated in the plating medium (Williams Medium E without phenol red supplemented with Hepatocyte Plating Supplement Pack)

The analysis was performed using a statistical software program (GraphPad, San Diego, CA, USA)

It prevents progression of anemia and nerve damage by supplying B directly, even when intrinsic factor (the gastric protein needed for oral B12 absorption) is lacking

More serious but less common risks include pancreatitis, gallbladder disease, and in people with type 2 diabetes potential worsening of diabetic retinopathy