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Src activation assay in HEK-293 arr1/arr2 dKO cells HEK-293 arr1/arr2 dKO cells 28 were co-transfected with receptor (FLAG-D1R or chimeric FLAG-2V2R), Src (only for chimeric FLAG-2V2R) and wild-type or mutant arr1 with a C-terminal HA tag with a 1:5 DNA:FuGENE6 (Promega) ratio according to the manufacturers instructions

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Maternal inheritance of glucose intolerance via oocyte TET3 insufficiency