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The redox potential of roGFP is dependent on the degree of oxidation ( OxD roGFP ) of the redox pair roGFP red /roGFP ox according to Equations 4 and 5: Substitution of Equation 5 into Equation 3 allows transformation of Equation 3 into Equation 6: OxD roGFP can be measured in vitro by monitoring the fluorescence emission intensities ( I ) at 520 nm for excitation at 390 and 480 nm
Similar change of pCav-1 was found, however, the total amount of Cav-1 was decreased
While it is naturally produced in our bodies, our levels decrease as we age, which contributes to changes in skin texture, the appearance of wrinkles, and shifts in skin color
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