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For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)

After the first puncture, bacteriostatic water is commonly stored at 28C (3646F) and used within the discard period specified on the manufacturer label, often around 28 days in clinical practice

This peptide is quick-acting, producing a rapid but relatively brief increase in GH levels

Each rabbit was observed individually in a controlled ground area for 20 minutes daily at a set time