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Total RNA was extracted from the hippocampal tissues using an RNA simple Total RNA kit (Tiangen Biotech Co., Ltd.), and the FastKing gDNA Dispelling RT SuperMix kit (Tiangen Biotech Co., Ltd.) was used to synthesize cDNA

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This is particularly important, considering that Pfizer (NYSE: PFE) recently discontinued its oral GLP-1 treatment because of these issues

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