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In three nested casecontrol studies from England and Wales, involving 440,089 patients with T2DM and without ASCVD treated with non-insulin glucose-lowering medication, combined SGLT2i and GLP-1RA therapy used for primary prevention was associated with 30% lower odds of MACE (defined as myocardial infarction/acute coronary syndrome, stroke/transient ischemic attack, and/or cardiovascular death) compared with other combination regimens (OR, 0.70

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Aging is associated with cardiac autonomic nerve fiber depletion and reduced cardiac and circulating BDNF levels

Every method requires disciplined sample handling, including immediate deproteinization, thiol alkylation, and strict temperature control, to prevent artifactual oxidation from compromising data integrity