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Levels of mouse cytokines were quantified in the culture supernatants by using IL-6, IFN-, IL-12p40 specific sandwich enzyme-linked immunosorbent assay (ELISA) following the manufacturers instructions (cytokine mouse uncoated ELISA kit, Invitrogen) as described 27

Copper(II) ions catalyze the oxidation of ascorbic acid through a redox reaction, rapidly degrading the vitamin C and potentially generating reactive oxygen species in the process

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