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A typical reaction solution was prepared by addition of GSH (100 l of the stock solution (3.0 mM) which gave a final concentration of 100 M) to phosphate buffer (3 ml) in a fluorescent cuvette
Thus, at a dose equal to one-half the LD 50 (5 mg/kg), less than 5% of the animals exposed to compound B would die, but 30% of the animals given compound A would die
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