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The preparation happens the night before, so there is zero effort when morning nausea makes cooking unappealing
Maher Jerudi, MD Clinical content is reviewed for accuracy and scope
To label these chimeric receptors at their C-termini with either NLuc or RLuc8, the plasmid was digested with HindIII and XbaI, and the purified fragment was ligated into either Arr3-NLuc or the GLP-1 receptor labeled at the C-terminus with RLuc8 (GLP-1RRLuc8) that had previously been digested with HindIII and XbaI, replacing the Arr3 or GLP-1R open reading frame with that of the chimeric receptor
5B, S6A-C and Supplementary Data 9)
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