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Total RNA was extracted from whole tick samples by homogenizing using an automill (Tokken, Chiba, Japan) and were added to TRI Reagent (Sigma-Aldrich)

If neurological symptoms were present at diagnosis, your doctor may perform a neurological examination at follow-up appointments to assess improvement

I also suggest a whole food, gluten-free, grain-free, sugar-free, dairy-free diet and avoid all processed foods