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We recommend that all hits should be resynthesised and purified to confirm activity but if this is not possible then compounds from commercial sources should be purified with metal scavenging technology before confirming activity
Enzymatic reactions were performed in 96-well plate format (Greiner Bio-One, 655101) with 190 l of reaction mix and 10 l of enzymes diluted in 10 mM sodium phosphate buffer pH 7.0 at the following concentrations: 1.36 g ml 1 (LacAnc100) and 0.81 g ml 1 (LaccID) for ABTS, 1.7 g ml 1 (LacAnc100) and 0.81 g ml 1 (LaccID) for DMP and guaiacol and 6.8 g ml 1 (LacAnc100) and 8.1 g ml 1 (LaccID) for sinapic acid
S.Krych.BuschardK.HansenC
[3] Dimercaptosuccinic acid or dimercaptopropane sulfonate may be used
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