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The bioavailability problem of berberine is widely recognized, and in the future its exact effect and long-term safety against DKD need to be more accurately assessed, and large-scale, high-quality preclinical and clinical studies must be conducted before clinical application

Samples were analyzed using accredited methods, including liquid chromatography coupled with high-resolution hybrid mass spectrometry and X-ray powder diffraction, to estimate PCT drug prevalence among other PED samples