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Doxorubicin resistant cells showed significantly increased RNA expression of anaplerotic genes pyruvate carboxylase, malic enzyme 1 (ME1), and malic enzyme 2 (ME2), corroborated by the enrichment of TCA cycle intermediates citrate, malate, and fumarate (Kim, 2018)

Follicular fluid IGF concentrations correlate with oocyte competence

Primers were designed to fit in resulting vector, pJB-HTS upstream of the hexa-histadine tag (5/phos/CCATGGGCAGCAGCCATCATCAT), and downstream (AGCTGGAATTCCTAGTTATTGCTCAGCGGTGGC) (Integrated DNA technologies) of the spacer yielding a fragment containing a phosphorylated 5 end, an initiation codon, a hexa-histadine tag, a thrombin cleavable sequence, a spacer, a termination codon, and an EcoRI site

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