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The PI4K do not appear to be capable of phosphorylating any other substrate besides PtdIns
Standard practice: inject BAC water slowly down the vial wall, allow 1015 minutes for complete dissolution, then swirl gently
After incubation of the supernatants with Escherichia coli NO 3 reductase (37, 30 min), 1 ml of Griess reagent (0.5% naphthylenediamine dihydrochloride, 5% sulfonilamide, 25% H 3 PO 4 ) was added
Using the calculator across escalation phases Here is a practical workflow: at the beginning of each 4-week escalation phase, open the calculator, enter your vial details and new dose, screenshot the results, and save it to your phone
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