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Previous studies suggested that patients with T2D typically experience lower %WL with GLP-1RAs, including semaglutide 2.4 mg compared with those with normoglycemia 22
Immunoblotting Proteins were extracted from tissues using radio-immunoprecipitation assay (RIPA) lysis buffer (#89900, Thermo Fisher Scientific) supplemented with protease and phosphatase inhibitor cocktails (#QTPPI1015, Bio-Rad, Hercules, CA, USA)
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