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2.9 Generation of reactive oxygen species Intracellular reactive oxygen species (ROS) production was assessed using the 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) assay ( g for 5 min at 4 C, washed to remove excess DCFH-DA, and resuspended in PBS to a final hematocrit of 10%
Hemil, H
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