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Champagne et al., J Am Diet Assoc 2002
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After spinning, supernatant was removed and pellet was resuspended in 0.1 M sodium cacodylate buffer (pH 7,4) + 2.5% glutaraldehyde and stored overnight at 4 C
Two days after plating, various concentrations of glucose, DCA, triplete, and 007-AM in modified Ringer buffer (RB: 140 mM NaCl, 3.5 mM KCl, 0.5 mM NaH 2 PO 4 , 1.5 mM CaCl 2 , 10-mM HEPES, and 2-mM NaHCO 3 ) containing DPP-4 inhibitor (25 M) and 0.5% (v/v) BSA were administered to the cells, which were then incubated for 2 h
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