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A total of 75 islets were loaded into individual chambers and perifused at a constant rate of 200 l min 1 using 2.7 mM glucose Krebs-Ringer-phosphate-HEPES (KRPH) buffer (140 mM NaCl, 4.7 mM KCl, 1.5 mM CaCl 2 , 1 mM NaH 2 PO 4 , 1 mM MgSO 4 , 2 mM NaHCO 3 , 5 mM HEPES and 0.1% FA-free BSA (pH 7.4)) with 100 l of Bio-Gel P-4 Media (Bio-Rad)

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Selected Research References Raun K, Hansen BS, Johansen NL, et al

Double-blind placebo-controlled study with PT-141 (MC4R-selective derivative of Melanotan II) 20 mg intranasally