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The mechanism appears to be interference with erythropoiesis
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Based on preclinical (animal and cellular) data only, inhibition of NNMT by 5-Amino-1MQ is theorised to: Raise intracellular NAD+ levels Activate SIRT1 and other sirtuins (proteins linked to metabolic regulation) Reduce fat cell (adipocyte) size and proliferation Improve insulin sensitivity These effects have been demonstrated in mouse models (Neelakantan et al., 2019), but robust human clinical trial data are currently absent
Cell culture studies In laboratory studies using 3T3-L1 adipocytes, a commonly used fat cell line, researchers found that 5-amino-1mq significantly reduced intracellular levels of 1-methylnicotinamide, confirming that it was successfully inhibiting NNMT
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